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1996-03-09
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Document 0201
DOCN M9650201
TI Prevalence of infection by human T-cell leukemia virus types I and II in
southern Spain.
DT 9605
AU Calderon EJ; Rey C; Medrano FJ; Sanchez-Roman J; Soriano V; Torres Y;
Ruiz M; Lissen E; Leal M; Department of Internal Medicine, Viral
Hepatitis and AIDS Study; Group, Virgen del Rocio University Hospital,
Sevilla, Spain.
SO Eur J Clin Microbiol Infect Dis. 1995 Aug;14(8):686-90. Unique
Identifier : AIDSLINE MED/96089502
AB To assess the spread of human T-cell leukemia virus (HTLV) type I and II
in different population groups at potential risk of infection in Spain,
a total of 756 subjects were studied: 453 belonging to groups at risk
for retrovirus infection, 255 with diseases potentially linked to
HTLV-I/II infection and 48 immigrants from endemic areas. An HTLV-I
viral-lysate enzyme immunoassay (EIA) with a recombinant transmembrane
envelope protein incorporated was used to screen serum samples. Reactive
specimens were confirmed by Western blot strips spiked with recombinant
proteins that differentiated HTLV-I from HTLV-II. Infection was then
verified by the polymerase chain reaction (PCR). Serum samples from 19
of the 756 subjects analyzed (2.5%) were reactive for HTLV by EIA. One
of these was from an intravenous drug user (IVDU) in whom HTLV-II
infection was confirmed by Western blot and PCR; a specimen from another
IVDU showed Western blot reactivity for both retroviruses, but PCR
results were negative. Lastly, Western blot confirmed the presence of
HTLV in one of the immigrant subjects. Western blot did not verify HTLV
infection in the remaining 16 cases, indicating a high rate of
nonspecific anti-HTLV reactivity when a second-generation EIA screening
test was applied. These results suggest that HTLV is present in Spain
among populations at high risk for HTLV, although at a very low rate and
restricted to intravenous drug users and individuals immigrating from
endemic areas.
DE Blotting, Western Human HTLV-I Infections/*EPIDEMIOLOGY/IMMUNOLOGY
HTLV-II Infections/*EPIDEMIOLOGY/IMMUNOLOGY Immunoenzyme Techniques
Polymerase Chain Reaction Prevalence Risk Factors Seroepidemiologic
Methods Spain/EPIDEMIOLOGY JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).